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- Primary Antibodies ›
- Caspase 3 Antibodies
Proteintech
View all (32) Caspase 3 antibodies
Cite Caspase 3 Monoclonal Antibody (2G4B2)
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Caspase 3 Antibody (66470-2-IG) in IHC (P)
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66470-2-IG ( CASP3 antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
66470-2-IG
Applications
Tested Dilution
Publications
Western Blot(WB)
1:1,000-1:6,000
View 172 publications172 publications
Immunohistochemistry (IHC)
-
View 16 publications16 publications
Immunohistochemistry (Paraffin) (IHC (P))
1:150-1:600
-
Immunohistochemistry (PFA fixed)(IHC (PFA))
1:50-1:500
-
Immunocytochemistry(ICC/IF)
1:50-1:500
View 14 publications14 publications
Not applicable(N/A)
-
View 2 publications2 publications
Product Specifications
Species Reactivity
Human,Mouse,Rat
Published species
Human,Mouse,Rat
Host/Isotype
Mouse/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2G4B2
Immunogen
Caspase 3 Fusion Protein Ag25029 (31-176 aa encoded by BC016926)
Conjugate
Unconjugated
- CoraLite Plus 488
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody can recognize p17, p19 and p32 of Caspase 3.
Immunogen sequence: ISLDNSYKM DYPEMGLCII INNKNFHKST GMTSRSGTDV DAANLRETFR NLKYEVRNKN DLTREEIVEL MRDVSKEDHS KRSSFVCVLL SHGEEGIIFG TNGPVDLKKI TNFFRGDRCR SLTGKPKLFI IQACRGTELD CGIETDS (31-176 aa encoded by BC016926)
Target Information
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases:Apopain; Apopain precursor; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase3; CPP-32; CPP32beta; Cysteine protease CPP32; ICE3; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; yama protein
Gene Aliases:A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama
UniProt ID:(Human) P42574, (Mouse) P70677, (Rat) P55213
Entrez Gene ID:(Human) 836, (Mouse) 12367, (Rat) 25402
Molecular Function: 
protease
Function(s)
response to hypoxiaB cell homeostasisapoptotic DNA fragmentationproteolysisapoptotic processactivation of cysteine-type endopeptidase activity involved in apoptotic processcellular component disassembly involved in execution phase of apoptosiscellular response to DNA damage stimulusheart developmentsensory perception of soundlearning or memoryactivation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome cresponse to UVresponse to glucoseresponse to X-rayregulation of macroautophagyprotein processinghippocampus developmentneuron differentiationkeratinocyte differentiationerythrocyte differentiationplatelet formationnegative regulation of B cell proliferationresponse to cobalt ionresponse to estradiolresponse to lipopolysaccharideglial cell apoptotic processresponse to tumor necrosis factorresponse to nicotinehippo signalingwound healingresponse to drugresponse to hydrogen peroxideT cell homeostasisnegative regulation of apoptotic processresponse to amino acidpositive regulation of neuron apoptotic processcell fate commitmentnegative regulation of cyclin-dependent protein serine/threonine kinase activitynegative regulation of activated T cell proliferationresponse to antibioticneurotrophin TRK receptor signaling pathwayresponse to glucocorticoidneuron apoptotic processcellular response to organic cyclic compoundapoptotic signaling pathwayextrinsic apoptotic signaling pathway in absence of ligandexecution phase of apoptosisresponse to woundingintracellular signal transductionpositive regulation of apoptotic processpositive regulation of catalytic activitynegative regulation of cell cyclecellular response to organic substancerelease of cytochrome c from mitochondriaextrinsic apoptotic signaling pathway via death domain receptorsintrinsic apoptotic signaling pathway in response to oxidative stressresponse to organic substanceresponse to metal ionresponse to organic cyclic compoundregulation of apoptotic DNA fragmentation
Process(es)
protease bindingaspartic-type endopeptidase activitycysteine-type endopeptidase activitycyclin-dependent protein serine/threonine kinase inhibitor activitydeath receptor bindingprotein bindingpeptidase activitycysteine-type endopeptidase activator activity involved in apoptotic processphospholipase A2 activator activityprotein complex bindingcysteine-type endopeptidase activity involved in apoptotic processcysteine-type endopeptidase activity involved in execution phase of apoptosiscysteine-type peptidase activityhydrolase activity
It has to be done as per old AB suggested Products section.
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